Crystalline Rhodanese. II. The Enzyme Catalyzed Reaction.
نویسندگان
چکیده
منابع مشابه
The Mechanism of the Rhodanese-catalyzed Thiosulf atekipoate Reaction
The individual reactions comprising the rhodanesecatalyzed thiosulfate-lipoate reaction were demonstrated kinetically. The mechanism was shown to be a reaction sequence in which the enzyme forms kinetically significant binary complexes with both thiosulfate and dihydrolipoate substrates. Moreover, the first product (sulfite) is discharged, forming a sulfur-substituted enzyme intermediate, befor...
متن کاملCrystalline beef kidney rhodanese.
Although rhodanese activity occurs in many tissues (l-3), the enzyme had been obtained in crystalline form from beef liver only (4). Beef kidney rhodanese could not be crystallized by the same procedure (5). To extend the biochemical genetic comparison reported previously (5), however, it was desired to obtain the kidney enzyme in a very high state of purity. This paper reports the purification...
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BACKGROUND Isothermal calorimetry allows monitoring of reaction rates via direct measurement of the rate of heat produced by the reaction. Calorimetry is one of very few techniques that can be used to measure rates without taking a derivative of the primary data. Because heat is a universal indicator of chemical reactions, calorimetry can be used to measure kinetics in opaque solutions, suspens...
متن کاملProteolytic interconversion of electrophoretic variants of the enzyme rhodanese.
It has been confirmed that the enzyme rhodanese, although a homogeneous single polypeptide chain protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, is separable by electrophoresis under nondenaturing conditions into four species which differ in net surface charge (I-IV in the order of increasing positive charge). Limited proteolysis can interconvert these species. Chymotrypsi...
متن کاملRhodanese-catalyzed reduction of thiosulfate by reduced lipoic acid.
by a double displacement mechanism (1, 2). Thiosulfate and organic thiosulfonates will act as sulfur donors for the enzyme (3). Cyanide ion, SOS=, and the organic sulfinates will accept sulfur from the enzyme-sulfur compound (1, 4, 5). In this study, crystalline beef liver rhodanese has been found to catalyze the reduction of SSOp to HSand SOf. Several powerful reducing agents, such as NaBH4 an...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1953
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.07-1137